Characterization of an industrially relevant carbohydrate oxidase from Microdochium nivale hos Københavns Universitet

The oxidase from Microdochium nivale is produced commercially by Novozymes A/S, and is used in the dairy industry to control production of lactic acid. The oxidase seems to operate in two different modes, probably controlled by a molecular “pH-switch”.

The aim of this project is to, firstly, confirm and characterize the changes in enzyme activity and substrate profile, upon changing pH. Secondly, to elucidate the complex structure-function relationships behind the switch. To facilitate the latter, several enzyme mutants with substitutions in relevant amino acid residues have been made available by Novozymes A/S.

The work will take place at the Frederiksberg Campus and will give the student an opportunity to learn an array of skills in enzyme chemistry. These include enzyme kinetics, structure analysis, HPLC, calorimetry, and other.

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